UCSF Navigation Bar

UCSF Departments of Pathology & Laboratory Medicine Home Page

Lab Manual for UCSF Clinical Laboratories

Lab Manual for SFGH

Internal Resources

DIFFERENTIAL LEUKOCYTE COUNT, MANUAL (INCLUDES PLATELET SCREEN AND RBC MORPHOLOGY

Item Value
Approval req'd? yes, except for 6H Nursery
Available Stat? Yes
Test code CPMD, (CBC + PLT + manual differential)
Performed by? Hematology
Sendout? no
Price range $$
In House Availability 24 hours/ 7 days

Add-ons are only accepted within 8 hours of specimen collection, as cell morphology deteriorates in older specimens.
Principle Microscopic examination of stained blood film. The relative proportion of each type of leukocyte is expressed in percent, based on visual examination of 100 cells. This procedure is limited by high inherent imprecision.
Container type EDTA (lavender top) tube
Amount to Collect 1 mL
Sample type Venous Blood
Normal range REFERENCE RANGES (MANUAL DIFFERENTIAL): ADULTS

Relative Ranges Conventional Units SI Units
Neutrophils 44-68% 0.44-0.68
Bands 0-6% 0.00-0.06
Lymphocytes 25-44% 0.25-0.44
Monocytes 0-7% 0.0-0.07
Eosinophils 0-4% 0.0-0.04
Basophils 0-2% 0.0-0.02


Calculation for Absolute Values: (WBC x %diff)/100 = Absolute Count


REFERENCE RANGES (MANUAL DIFFERENTIAL): PEDIATRICS

(Percentage Values)

Age (103/mcL)
WBC
(%)
PMN
(%)
BAND
(%)
LYMP
(%)
MONO
(%)
EOS
(%)
BASO
Birth 9.0-30.0 51-71 0-4 21-41 2-10 0-4 0-2
12 hrs 13.0-38 58-78 0-4 16-32 1-9 0-4 0-2
24 hrs 9.4-34.0 51-71 0-4 21-41 2-10 0-4 0-2
2-7 days 5.0-21.0 35-55 0-4 31-51 3-15 0-8 0-2
8-14 days 5.0-20.0 30-50 0-4 38-58 3-15 0-7 0-2
15-30 days 5.0-19.5 25-45 0-4 46-66 1-13 0-7 0-2
1-6 mos 5.0-19.5 22-45 0-4 46-71 1-13 0-7 0-2
0.5-1 yr 6.0-17.5 21-42 0-4 51-71 1-9 0-7 0-2
1-2 yrs 6.0-17.5 21-43 0-4 49-71 1-9 0-7 0-2
2-4 yrs 5.5-17.0 23-52 0-4 40-69 1-9 0-7 0-2
4-6 yrs 5.0-15.5 32-61 0-4 32-60 1-9 0-7 0-2
6-8 yrs 4.5-14.5 41-63 0-4 29-52 0-9 0-7 0-2
8-10 yrs 4.5-13.5 43-64 0-4 28-49 0-8 0-4 0-2
10-16 yrs 4.5-13.5 44-67 0-4 25-48 0-9 0-7 0-2
16-18 yrs 3.9-13.0 44-68 0-6 25-48 0-9 0-7 0-2


(Absolute Values)

Age (103/mcL)
WBC
(103/mcL)
PMN
(103/mcL)
BAND
(103/mcL)
LYMP
(103/mcL)
MONO
(103/mcL)
EOS
(103/mcL)
BASO
Birth 9.0-30.0 4.6-21.0 0.0-1.2 1.9-2.3 0.2-3.0 0.0-1.2 0.0-0.6
12 hrs 13.0-38 7.5-14.4 0.0-1.5 2.1-12.2 0.1-3.4 0.0-1.5 0.0-0.8
24 hrs 9.4-34.0 4.8-24.0 0.0-1.4 2.0-13.9 0.2-3.4 0.0-1.4 0.0-0.7
2-7 days 5.0-21.0 1.8-11.0 0.0-0.8 1.6-10.7 0.2-3.2 0.0-1.7 0.0-0.4
8-14 days 5.0-20.0 1.5-10.0 0.0-0.8 1.9-11.6 0.2-3.0 0.0-1.4 0.0-0.4
15-30 days 5.0-19.5 1.3-8.0 0.0-0.8 2.3-12.9 0.5-2.5 0.0-1.4 0.0-0.4
1-6 mos 5.0-19.5 1.1-8.8 0.0-0.8 2.3-13.8 0.1-2.5 0.0-1.4 0.0-0.4
0.5-1 yr 6.0-17.5 1.3-7.4 0.0-0.7 3.1-12.4 0.1-1.6 0.0-1.2 0.0-1.2
1-2 yrs 6.0-17.5 1.3-7.5 0.0-0.7 2.9-12.4 0.1-1.6 0.0-1.2 0.0-1.2
2-4 yrs 5.5-17.0 1.3-8.8 0.0-0.7 2.2-11.7 0.6-1.5 0.0-0.5 0.0-0.3
4-6 yrs 5.0-15.5 1.6-9.5 0.0-0.6 1.6-9.3 0.5-1.4 0.0-1.1 0.0-0.3
6-8 yrs 4.5-14.5 1.9-9.1 0.0-0.6 1.3-7.5 0.0-1.3 0.0-1.0 0.0-0.3
8-10 yrs 4.5-13.5 1.9-8.6 0.0-0.5 1.3-6.6 0.0-1.1 0.0-0.5 0.0-0.3
10-16 yrs 4.5-13.5 2.0-9.1 0.0-0.5 1.1-6.5 0.0-1.2 0.0-1.0 0.0-0.3
16-18 yrs 3.9-13.0 1.7-8.0 0.0-0.8 1.0-6.2 0.0-1.2 0.0-0.9 0.0-0.3


Modified from: Dallman, P.R.: In Pediatrics, 16th ed. Rudolph, A.[editor], New York, Appleton Century Crofts, Inc., 1977:1178. Nathan D.G., Orkin S.H.: Nathan and Oski's Hematology of Infancy and Childhood, 5th ed. Philadelphia, WB Saunders Co., 1998.


Synonyms Platelet Screen;
Additional information INDICATIONS:

1. Detection of morphologic abnormalities of WBC, RBC, and platelets;

2. Evaluation of qualitative abnormalities observed on an automated blood count.


References Rodale, BF (ed). Hematology – Clinical Principles and Applications, 2nd ed. Philadelphia, WB Saunders Co., 2002
CPT coding 85007
Last Updated 5/27/2011 4:29:12 PM
Search Our Test Menu
UCSF home page UCSF home page About UCSF Search UCSF UCSF Medical Center