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DIFFERENTIAL LEUKOCYTE COUNT, AUTOMATED

Item Value
Approval req'd? no
Available Stat? Yes
Test code CDP (CBC+ DIFF+ PLT)
Performed by? Hematology
Sendout? no
Price range $
In House Availability 24 hours/ 7 days

Principle A diluted blood sample is examined by automated cell/light scatter analysis and flow cytometry and counted. Precision for total granulocyte and lymphocyte counts is at least ten times better than that of the traditional manual differential.

Container type EDTA (lavender top) tube
Amount to Collect 1 mL
Sample type Venous Blood
Normal range REFERENCE RANGES, ADULTS (6 part automated differential). See Differential Leucocyte Count, Manual, for pediatric reference ranges.

RELATIVE
RANGES: Conventional Units SI Units
Neutrophils 44 - 68% 0.44 - 0.68
Lymphocytes 25 - 44% 0.25- 0.44
Monocytes 0.0 - 7% 0.0 - 0.07
Eosinophils 0.0 - 4% 0.0 - 0.04
Basophils 0.0 - 2% 0.0 - 0.02
Large lymph 0.0 - 4.5% 0.0 - 0.045

ABSOLUTE
RANGES: Conventional Units SI Units
Neutrophils 1.7 - 8.0 x103/mcL 1.7 - 8.0 x 109/L
Lymphocytes 1.0 - 5.1 x103/mcL 1.0 - 5.1 x 109/L
Monocytes 0.0 - 0.8 x103/mcL 0.0 - 0.8 x 109/L
Eosinophils 0.0 - 0.5 x103/mcL 0.0 - 0.5 x 109/L
Basophils 0.0 - 0.2 x103/mcL 0.0 - 0.2 x 109/L
Large lymph 0.0 - 0.5 x103/mcL 0.0 - 0.5 x 109/L

Stability Stable for 24 hours at 20-30°C (Eosinophils stable for 8 hrs).
References 1. Miers, MK, et al: Cell Counting Instrumentation. In: Hematology – Clinical Principles and Applications, 2nd ed. Rodale, BF (ed). Philadelphia, WB Saunders Co, 2002.

2. Samiy, A.H. Textbook of Diagnostic Medicine, Philadelphia, Lea & Febiger, 1987: 845 858.

3. Var, D et al.: Reference Intervals for a Complete Blood Count Determined on Different Automated Haematology Analyzers: Abx Pentra 120 Retic, Coulter Gen-S, Sysmex SE 9500, Abbott Cell Dyn 4000 and Bayer Advia 120 Clin Chem Lab Med. 2002 40(1): 69-73.
CPT coding 85025
Last Updated 5/27/2011 4:26:57 PM
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