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Lab Manual for UCSF Clinical Laboratories

Lab Manual for SFGH

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CYTOMEGALOVIRUS (CMV) ISOLATION

Item Value
Available Stat? No
Performed by? Reference Laboratory
Sendout? yes
Price range $$$
In House Availability 7 days
Principle Specimens for detection of cytomegalovirus are inoculated to appropriate cells in viral shell vials and stained for CMV early antigen after 24 and 48 hours. These specimens are also inoculated to appropriate cell lines, which are examined for appearance of cytopathic effect (CPE). Identity of viruses is made through recognition of typical CPE and confirmation by fluorescent antibody staining. Cultures are held for 2 weeks before being reported as "Negative."
Method VIRCM
Interpretation INTERPRETATION: Positive result indicates the presence of CMV, but does not necessarily confirm etiologic relationship with existing disease. CMV shedding may persist for weeks to years after primary infection.

Negative result may indicate absence of virus or may be due to improper specimen collection, improper specimen handling, or small amounts of virus which were not detected.

CLINICAL SIGNIFICANCE: The result must be interpreted in conjunction with clinical findings and other laboratory data.
Container type 1. Use swabs provided with viral transport medium (obtained from Lab Support Services, 206-8199) for collecting specimens which require a swab. If viral transport packages are not available, dacron swabs with plastic or fine-wire shafts may be used and placed into a small amount (approx. 0.5 mL) of sterile non-bacteriostatic saline in a sterile tube for transport to the lab. Dry swabs will not be processed. Do not use calcium alginate swabs.

2. Put other specimens for culture in sterile, leak-proof containers.
Collection Instructions Collect specimens aseptically as soon as possible after onset of symptoms or at autopsy.

For detailed collection instructions for specific specimen types, see Virology, Routine Culture.
Special instructions Deliver specimens to Microbiology Lab (2M33) immediately after collection or hold at 4°C until delivery.
Normal range No virus recovered.
Synonyms Autopsy Viral Culture;BAL (Bronchoalveolar Lavage) Culture;Biopsy Culture;Bronchoalveolar Lavage (BAL) Culture;CMV Isolation;
Stability CMV is a relatively labile virus. For optimal isolation, bring specimens to the laboratory immediately after collection.
Additional information Blood cultures for CMV are not available. See Cytomegalovirus (CMV), PCR on Blood or Bone Marrow.

REQUISITION: Microbiology (Viral). Indicate clearly on requisition that specimen is to be cultured for CMV. If CMV is not indicated on requisition, only routine viral isolation may performed, and CMV may not be recovered.
References 1. Clarke, LM, ed. 1992. Section 8: Viruses, Rickettsiae, Chlamydiae, and Mycoplasmas; as found in Clinical Microbiology Procedures Manual, H. Isenberg, ed. Amer. Soc. for Microbiol., Washington, D.C.

2. Schmidt, NJ and RW Emmons, eds. 1989. Diagnostic Procedures for Viral, Rickettsial and Chlamydial Infections, 6th ed. Amer. Public Health Assn, Washington, D.C.
Last Updated 8/19/2009 1:20:56 PM
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