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Lab Manual for UCSF Clinical Laboratories

Lab Manual for SFGH

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AMPHETAMINE, METHAMPHETAMINE, ECSTASY, URINE

Item Value
Available Stat? Yes
Test code AMPHP
Performed by? Chemistry
Sendout? no
Price range $$$
In House Availability Routine or Stat: 24 hours, 7 days
Principle Competitive immunoassay using CEDIA technology
Interpretation The amphetamines are central nervous system stimulants that can lead to aggressive behavior.

CUTOFF: 1000 ng/mL Drug Detection Period (approximate guideline) = 2-4 days.

Positive screening results are reported as "Preliminary Positive - confirmation will be performed" and specimens are referred automatically for confirmation testing and identification of the specific agent using LC-MS/MS. Drugs detected include: amphetamine, methamphetamine, methylene dioxymethamphetamine (MDMA) and metabolite (MDA). At SFGH, 90% of positive screens for amphetamines are confirmed.
Amount to Collect 10 mL
Sample type Urine
Stability Minimum 1 week at 2-8°C
Interferences This assay has low cross-reactivity towards other amines. Some pharmaceutical drugs, such as bupropion, trazadone, sildenafil, and aripiprzole can cause a positive screen at high concentrations. This assay will cross-react with a subset of designer drugs (i.e., bath salts), but not all of them. Substituted amphetamines (i.e. 4-fluoroamphetamine and para-methoxyamphetamine) and piperazines (benzylpiperazine and chlorophenylpiperazine) show moderate to significant cross-reactivity. Other stimulants, such as ephedrine, pseudoephedrine, phenylpropanolamine, methylphenidate and phenylephrine, should not cross-react with this assay at physiologically relevant concentrations.
Turn around times Screening results are reported within 1 to 8 hours. Confirmation results will be reported as a separate test when they are completed (1-4 days).
References 1. Wu AHB, Broussard LA, Hoffman RS, Kwong TC, McKay C, Moyer TP, Otten EM, Welch SL, Wax P. National Academy of Clinical Clinical Chemistry. Laboratory Medicine Practice Guidelines. Recommendations for the Use of Laboratory Tests to Support the Impaired and Overdosed Patients from the Emergency Department. Clin Chem 2003;49:357-79.
Last Updated 7/24/2015 3:16:29 PM
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