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Lab Manual for UCSF Clinical Laboratories

Lab Manual for SFGH

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ALT (Alanine AminoTransferase)

Item Value
Available Stat? Yes
Test code ALT
Performed by? Chemistry
Sendout? no
Price range $
In House Availability Routine: 7 days
Principle ALT is measured kinetically with detection based on the oxidation of NADH by lactate dehydrogenase.


                                  ALT
2-ketoglutarate + L-alanine       <->         glutamate + pyruvate

                                  LDH
pyruvate + NADH + H               <->         lactate + NAD+

Interpretation ALT is found in highest concentrations in liver and renal tissues but is also found in heart, skeletal muscle, pancreas, spleen, and lung. Increased levels reflect acute hepatic disease more specifically than AST. Greatly elevated ALT levels occur in infections or toxic hepatitis, infectious mononucleosis, and cirrhosis. This method measures total serum ALT irrespective of its tissue of origin. Other tests may be necessary to aid in differential diagnosis.
Container type gel tube (gold or green top)
Amount to Collect 2 mL
Sample type Blood
Special instructions Moderately hemolyzed serum will not be assayed.
Normal range Male 10 - 40 U/L Female 7 - 35 U/L
Synonyms Liver Panel (ALB, ALT, AST, ALKP, TP, TBIL, DBIL);Metabolic Comprehensive Panel (METC): METB, TBIL, TP, ALB, ALT, AST, ALKP;
Stability Serum is stable 24 hrs at room temperature, 4°C, or -20°C
Interferences Increased values: gross hemolysis.
References 1. Rodgerson, D.O., Osbeg, I.M.: Sources of error in spectrometric measurement of aspartate aminotransferase and alanine aminotransferase activities in serum. Clin Chem, 20:43, 1974.

2. Bayer(TM) Clinical Methods ALT (56) B01-4140-01 Advia 1650 Operator's Guide. PN 073-5001-09. Last accessed June 2003. Siemens Medical Solutions Diagnostics, Malvern, PA.

3. Tietz, N.W. Textbook of Clinical Chemistry and Molecular Diagnosis, ed. 4. St. Louis, MO: Elsevier Saunders, 604-607; 2006.
CPT coding 84460
Last Updated 12/29/2008 2:02:55 PM
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