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Lab Manual for UCSF Clinical Laboratories

Lab Manual for SFGH

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BACTERIA CULTURE, STOOL

Item Value
Available Stat? No
Test code STLC, STNS
Performed by? Microbiology (Bacteriology)
Sendout? no
Price range $$$-$$$$
In House Availability 7 days
Principle Specimens for stool cultures should be obtained as soon as possible when enteric infection is suspected. Follow-up cultures may be obtained after start of treatment.

The laboratory routinely looks for Salmonella, Shigella, E. coli O157 and Campylobacter jejuni-coli. In addition, when requested, the Laboratory will set up special culture media to look for the following enteric pathogens:

1. Vibrio cholerae and sea water vibrios (V. vulnificus, V. parahaemolyticus [TCBS Agar])
2. Yersinia enterocolitica causes enterocolitis and mesenteric adenitis (CIN or Yersinia Agar).

Positive results are phoned to the physician. Negative culture final reports are issued in LCR after three days.
Container type Para-Pak C&S Vial (Cary Blair transport medium)
Amount to Collect 5 mL
Collection Instructions Collect stool in Para-Pak C&S vial, available through Lab Support Services (415-206-8199). Multi-language directions in the collection kit describe proper collection.

If stool is submitted in sterile, screw cap container, it must be received in the Microbiology Laboratory within 2 hours of collection.
Sample type Stool
Min. Volume 5 mL
Special instructions Multi-language directions describe proper collection. If fresh, unpreserved specimen is submitted, it must be received in the Microbiology Laboratory within 2 hours or it will not be processed.
Normal range No Salmonella, Shigella, E. coli O157, or Campylobacter jejuni-coli recovered.
Synonyms Salmonella; Shigella; Campylobacter; E. coli O157:H7; Enterohemorrhagic E. coli (O157:H7); Stool Culture; Yersinia enterocolitica; Vibrio
Stability Unpreserved: 2 hours.
Preserved in Para-Pak C&S vial and refrigerated 2° - 8°C: 3 days.
Causes for rejection 1. Unpreserved stool received > 2 hours after collection.
2. Stool in Para-Pak C&S vial > 72 hours after collection.
3. More than one sample received per 24 hour period.
Turn around times 4 - 5 days
Additional information Shiga toxin assay is automatically performed on stools submitted for bacterial stool culture, and is billed separately.

Do not send a stool specimen for culture in an SAF vial. The SAF vial is for Stool Parasitology only. (See Parasitology).

C. difficile is not cultured. If C. difficile infection is suspected, submit stool specimen in sterile, screw-cap container and request the C. difficile Toxin B Gene Assay.
References 1. Clinical Microbiology Procedures Handbook, 3rd Ed., HD Isenberg Ed., Amer. Soc. for Microbiol., Washington DC, 2010.

2. Forbes, BA, et al. Bailey and Scott's Diagnostic Microbiology, 12th Ed., Mosby, St. Louis MO, 2007.
CPT coding Muliple CPTs
Last Updated 1/26/2016 8:48:56 AM
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