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Lab Manual for UCSF Clinical Laboratories

Lab Manual for SFGH

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BACTERIA CULTURE, GENITO-URINARY (GU)

Item Value
Available Stat? No
Test code GUC, GUCA
Performed by? Microbiology (Bacteriology)
Sendout? no
Price range $$$
In House Availability 7 days
Principle The specimen is inoculated onto plate and broth media, including a medium selective for Neisseria gonorrhoeae and when appropriate, anaerobe cultures will be done. Surgical laparoscope, protected catheter or culdocentesis collected specimens are necessary for anaerobe culture. Vaginal and cervical os collections normally have a large anaerobic bacterial flora and are not satisfactory for anaerobe culture. See Anaerobic Cultures (Microbiology Procedures Section III-B-2) for additional information.
Interpretation Cultures are appropriate for diagnosis of endometritis, pelvic inflammatory disease, infected abortion or gonorrhea. Bacteria isolated from female upper genital tract specimens may be a result of contamination of these specimens with cervical/vaginal flora.
Collection Instructions SEE THE INDIVIDUAL COLLECTION INSTRUCTIONS REGARDING PROSPECTIVE DIAGNOSIS.

1. Culdocentesis, cul de sac fluid or laparoscopic specimen: 1-2 mL fluid in sterile or anaerobe transport tube. Keep specimen at room temperature until delivery to the Laboratory.

2. Endocervix or vaginal: 2 swabs in transport medium or lightly moistened calcium alginate swabs in sterile tube. Deliver to lab rapidly to prevent lethal drying. Keep specimen at room temperature until delivery to the Laboratory.

3. Urethra: Calcium alginate swab lightly moistened in sterile tube. Collect at least one hour after urination. Keep specimen at room temperature until delivery to the Laboratory.

4. Genital Tract Tissue: Send small piece of tissue, collected in surgery or by device in protective catheter, in sterile cup or anaerobe transport tube. Keep specimen at room temperature until delivery to the Laboratory.

5. Culture when gonorrhea is suspected. It is preferable to inoculate part of the specimen directly to a Thayer Martin plate (warmed to room temperature). Either hold plates in candle jar or deliver immediately to the Microbiology Lab (2M33). Neisseria gonorrhoeae does not survive well without increased carbon dioxide and is also highly susceptible to drying and extremes of temperature.
Special instructions It is essential to indicate prospective diagnosis so that specimen is processed correctly.
Synonyms Bacteria Culture, Cervical, Vaginal, and GU;Neisseria Gonorrhoeae Culture;
Additional information (Also see GC Only Cultures & Diagnostic Tests for Chlamydia Infection)
References Murray, PR et al., Manual of Clinical Microbiology, 9th Ed., ASM Press, Washington, DC, 2007.
CPT coding 87070
Last Updated 12/29/2008 3:06:01 PM
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