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Lab Manual for UCSF Clinical Laboratories

Lab Manual for SFGH

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RUBELLA VIRUS ISOLATION

Item Value
Approval req'd? yes, Microbiology Laboratory Medicine Resident
Available Stat? No
Performed by? Reference Laboratory
Sendout? yes
Price range $$$
In House Availability Attempts to isolate rubella virus may be done only when:
1. An infant is suspected of having congenital rubella, and
2. Documentation of rubella serology status of the mother during pregnancy is available.

Before collecting specimens, arrangements must be made with the Microbiology Lab (206-8576).
Principle Specimens are sent to California Department of Health Services for rubella virus detection.
Method VIRC
Collection Instructions 1) For throat swab, use viral transport package (swabs, transport medium). Rub tonsillar or posterior nasopharynx area vigorously. If viral transport package is not available, swab area thoroughly with 2 pre-moistened Dacron or cotton swabs. Place swabs into approximately 0.5 ml non-bacteriostatic saline. Dry swabs will not be processed. DO NOT USE CALCIUM ALGINATE SWABS.

2) For urines, collect 10 – 20 mL of clean voided urine in sterile screw-capped container.

3) For serum, collect 0.5 mL blood (NO anticoagulant) in heel-stick tube.
Sample type Throat swab, urine, or serum.
Special instructions Deliver to Microbiology Lab (2M33) immediately or hold at 4°C until delivery
Normal range No virus recovered
Additional information REQUISITION: Microbiology (Viral)
References Schmidt, NJ and RW Emmons, eds. 1989. Diagnostic Procedures for Viral, Rickettsial and Chlamydial Infections, 6th ed. Amer. Public Health Assn, Washington, D.C.
CPT coding 87252
Last Updated 8/23/2010 8:30:35 AM
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