UCSF Navigation Bar

UCSF Departments of Pathology & Laboratory Medicine Home Page

Lab Manual for UCSF Clinical Laboratories

Lab Manual for SFGH

Internal Resources

BACTERIA CULTURE, ANAEROBIC, ABSCESS / EXUDATE /ULCER /WOUND

Item Value
Available Stat? No
Test code MSA
Performed by? Microbiology (Bacteriology)
Sendout? no
Price range $$$
In House Availability 7 days
Principle Anaerobic infections often contain polymicrobic mixtures of aerobic and anaerobic bacteria. Anaerobic bacteria grow in reduced or poorly oxygenated environments such as necrotic, crushed, or devascularized tissue. Large inocula of anaerobes or facultative bacteria produce a site with a low redox potential (reduced) where anaerobes can survive and grow. Anaerobic bacteria may produce virulence factors that are responsible for tissue necrotizing lesions. A mixture of organisms may act synergistically to produce infection.
Interpretation Although anaerobes are part of normal oropharyngeal, gut and urogenital bacterial flora and are thought to play a significant role in the resistance against colonization by pathogenic organisms, they may also cause severe, lethal infections in humans when they gain access to normally sterile body sites. Infecting anaerobes may also be acquired from exogenous sources which produce toxigenic infections such as tetanus or botulism.
Container type carbon dioxide-filled anaerobe collection tube.
Collection Instructions See Microbiology Procedure Section III-B-2 for proper collection procedures and acceptable specimens for anaerobic culture.

Send properly obtained specimens for anaerobic culture in a carbon dioxide-filled anaerobe collection tube.

Swabs in transport medium are not satisfactory for the isolation of anaerobes and will not be processed for such. Only aerobic culture will be done on swabs.
Synonyms Abscess Culture;BACTERIA CULTURE, ANAEROBES;Ulcer, Culture;
Stability Keep anaerobe collection tube at room temperature - Do Not Refrigerate. Send to Microbiology Laboratory (2M33) as soon as possible.
References 1. Forbes, BA et al., Bailey and Scott's Diagnostic Microbiology, 11th Ed., Mosby, St. Louis MO, 2002.

2. Murray, PR et al., Manual of Clinical Microbiology, 9th Ed., ASM Press, Washington, DC, 2007.

3. Rodloff, AC et al., Cumitech 5A: Practical Anaerobic Bacteriology, Amer. Soc. for Microbiol., Washington DC, 1991.
CPT coding 87075
Last Updated 12/29/2008 2:46:08 PM
Search Our Test Menu
UCSF home page UCSF home page About UCSF Search UCSF UCSF Medical Center