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Lab Manual for UCSF Clinical Laboratories

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MYCOBACTERIA CULTURE, URINE

Item Value
Available Stat? No
Test code AFNS
Performed by? Microbiology (AFB)
Sendout? no
Price range $$$
In House Availability 7 days
Principle Specimen is concentrated by centrifugation. The sediment is decontaminated in order to prevent overgrowth by other organisms. An acid-fast smear is not performed on urine sediment because it may contain non-pathogenic mycobacteria which would appear acid-fast on a smear. The sediment is inoculated into AFB culture media and incubated for 8 weeks. Negative results are reported after 8 weeks. Positive results are reported when AFB are recovered.
Container type large volume, sterile, screw-capped container. Obtain the special urine AFB culture containers from Laboratory Support Services (206-8199).
Amount to Collect entire first morning voided urine
Collection Instructions Entire first morning voided urine collected by the clean-catch procedure in the special sterile screw-cap container, obtained from Laboratory Support Services (206-8199). Organisms accumulate in the bladder overnight, so the entire first morning voided urine provides the best yield. Specimens collected at other times are dilute and not optimal. The entire first morning voided urine is the most satisfactory specimen; supra-pubic aspirate, ureter urine and any urine from a baby is acceptable.
Sample type Urine
Min. Volume 40 mL
Synonyms Urine Culture;
Stability Deliver to Microbiology Laboratory (2M33) as soon as possible or hold at 4°C until delivery.
Additional information Contact the Laboratory Medicine Resident in Microbiology (206-8576 or pager 415-443-1438) for consultation if voided urine cannot be obtained.
References 1. Isenberg, HD (ed), Clinical Microbiology Procedures Handbook, Vol. 1, ASM Press, Washington DC, 1992.

2. Kent PT and Kubica GP. 1985. Public Health Mycobacteria: A Guide for the Level III Laboratory. U.S. Department of Health and Human Services. CDC. Atlanta GA.

3. Murray PR, et al. Manual of Clinical Microbiology, 8th ed. ASM Press, Washington, DC. 2003.
Last Updated 5/2/2011 5:13:19 PM
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