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Lab Manual for UCSF Clinical Laboratories

Lab Manual for SFGH

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MYCOBACTERIA, ACID-FAST BACILLI (AFB) STAIN

Item Value
Available Stat? No
Test code MC, AFSM
Performed by? Microbiology (AFB)
Sendout? no
Price range $$$
In House Availability 7 days
Principle Acid-fast smears are routinely made after a decontamination/concentration procedure. Smears are stained with the auramine rhodamine acid-fast stain. Smear results for specimens received in the Microbiology Laboratory by 4:00 PM are available by 11:00 AM the following morning. Housestaff should mark the third (3rd) sputum sample as "sample #3." Smear results for these samples will be available by 11:00 PM on the day of receipt.

Positive acid-fast smears prepared using the decontamination/concentration procedures are quantitated according to the following scale:

* Rare (3-9 per slide) acid-fast bacilli seen
* Few (10 or more per slide) acid-fast bacilli seen
* Numerous (1 or more per oil immersion field) acid-fast bacilli seen

All new positive smears are reported to the Infectious Diseases Fellow by phone. All positive smears are reported by written report.
Synonyms AFB Smears
References Kent, PT and GP Kubica. 1985. Public Health Mycobacteria: A Guide for the Level III Laboratory. U.S. Department of Health and Human Services. CDC. Atlanta GA.
CPT coding 87206
Last Updated 8/19/2009 1:31:17 PM
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