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Lab Manual for SFGH

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LH (LUTEINIZING HORMONE)

Item Value
Available Stat? No
Test code LH
Performed by? Chemistry
Sendout? no
Price range $$$
In House Availability Monday - Friday
Principle The Siemens Centaur assay is a two-site sandwich immunoassay using direct chemiluminometric technology
Interpretation LH is a glycoprotein hormone secreted by the anterior pituitary gland that stimulates gonadal development, production of steroidal sex hormones and gametogenesis. In the post-pubertal female, both gonadotrophins (LH, FSH) peak at midcycle, and are responsible in part for elevations in both progesterone and estrogen during the latter half of the cycle. Elevated levels of LH are seen in primary amenorrhea due to gonadal dysgenesis, such as Turner's syndrome.

After menopause, LH levels rise but may fluctuate widely over time.

In males, elevated LH levels are seen with primary hypogonadism, such as with Kleinfelter's Syndrome, whereas pituitary causes of hypogonadism are associated with decreased LH/FSH levels.
Container type gold top gel tube
Amount to Collect 2 mL
Collection Instructions Plasma samples cannot be assayed.
Sample type Blood
Normal range IU/L (mIU/mL)*
Male, < 50 years: 1.5 - 9.3
Adult Female: Follicular: 1.9 - 12.5
Ovulatory: 8.7 - 76.3
Luteal: 0.5 - 16.9
Post Menopausal: 15.9 - 54.0


*Units are referenced to Second International Standardized (2nd IS) 80/552 for LH.
Synonyms LH;
Stability Serum stable 48 hrs at 2-8°C; 1 month frozen at -20°C
Interferences 1. Oral contraceptives or estrogen replacement drugs that contain estrogen and/or progesterone may cause a physiologic decrease in serum levels of LH.

2. Auto- (rheumatoid factor) and heterophile antibodies in human serum can react with reagent immunoglobulins, interfering with in vitro immunoassays. Patients routinely exposed to animals or to animal serum products can be prone to this interference. Anomalous values may be observed. The most common, although not exclusive, interference seen in immunometric ("two-site," "sandwich") assays is a spuriously high value. Results inconsistent with clinical findings or previous laboratory values can be investigated by the laboratory for this phenomenon. Call the Clinical Chemistry Laboratory Medicine Resident (x65527, pager 415-443-2311).

3. A paradoxical, false low result may rarely occur when patient samples with extremely high concentrations of peptide/protein analytes (e.g. AFP, hCG, PSA, etc.) are measured by immunometric ("two-site," "sandwich") assays. For example, a true value of 3,000,000 U/L could be reported as < 5 or 21 U/L. This phenomenon is called the "high dose hook effect." Results inconsistent with clinical findings or previous laboratory values can be investigated by the laboratory for this phenomenon. Call the Clinical Chemistry Laboratory Medicine Resident (x65527, pager 415-443-2311).
References 1. Bayer 111736 Rev. E, 11/2000. Siemens Medical Solutions Diagnostics, Malvern, PA.

2. Tietz NW (ed). Fundamentals of Clinical Chemistry. Ed. 5. Philadelphia: WB Saunders, 377-378, 2001.
CPT coding 83002
Last Updated 6/18/2011 8:12:47 AM
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