UCSF Navigation Bar

UCSF Departments of Pathology & Laboratory Medicine Home Page

Lab Manual for UCSF Clinical Laboratories

Lab Manual for SFGH

Internal Resources

B-TYPE NATRIURETIC PEPTIDE (BNP)

Item Value
Available Stat? Yes
Test code BNP
Performed by? Chemistry
Sendout? no
Price range $$
In House Availability Stat: 24 hours, 7 days
Principle The Siemens Centaur assay is a two-site sandwich immunoassay using direct chemiluminometric technology.
Interpretation BNP is released from the ventricles of the heart in clinical conditions related to stretching and volume overload. In patients who present with shortness of breath and dyspnea, BNP measurements are used to assist in the diagnosis of congestive heart failure. BNP levels correlated to the severity of heart failure, as assessed by the New York Heart Association Classification (see table). High concentrations of BNP in patients with heart failure or ischemic heart disease correlate to a higher risk for cardiac death.

The following table shows ranges and median values (pg/mL) for BNP in patients in the different NYHA classifications of heart failure.

Classification 5th to 95th Percentile Median
NYHA I 15 to 499 95
NYHA II 10 to 1,080 222
NYHA III 38 to > 1,300 459
NYHA IV 147 to > 1,300 1,006
All CHF 22 to > 1,300 360




Container type plastic EDTA tube (white top); plasma from lavendar top tube acceptable, glass tube not acceptable
Amount to Collect 2 mL
Sample type Plasma
Special instructions Glass tube not acceptable. Mix by inversion 8 – 10 times. Do not centrifuge.
Normal range < 100 pg/mL
Synonyms BNP;
Stability Plasma; 1 week @ 4°C
Uncentrifuged PPT: stable 24 hours @ RT or 4°C
Interferences This assay does not cross-react with other peptides that have similar structures to BNP (e.g., ANP, CNP, NT-proBNP).
References 1. Maisel AS, Krishnaswamy P, Nowak RM, McCord J, Hollander JE, Duc P, Omland T, Storrow AB, Abraham WT, Wu AHB, et al. Rapid measurement of B-type natriuretic peptide in the emergency diagnosis of heart failure. N Engl J Med 2002; 347:161-7.

2. Wu AHB Packer M, Smith A, Bijou R, Fink F, Mair J, Wallentin L, Feldcamp CS, Haverstick DM, Ahnadi CE, Grant A, Despres N, Bluestein B, Ghani F. Analytical and clinical evaluation of the Bayer ADVIA Centaur automated B-type natriuretic peptide assay in patients with heart failure: a multisite study. Clin Chem 2004, 50: 867-73.

3. Bayer(TM) Clinical Methods B-type natriuretic peptide. Advia Centaur Operator's Guide. PN 02816634. Last accessed Oct 2004. Siemens Medical Solutions Diagnostics, Malvern, PA.
CPT coding 83880
Last Updated 10/15/2012 7:42:47 PM
Search Our Test Menu
UCSF home page UCSF home page About UCSF Search UCSF UCSF Medical Center