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INSULIN

Item Value
Available Stat? No
Test code INSU
Performed by? Chemistry
Sendout? no
Price range $$
In House Availability Mon - Fri; Sat & Sun (12 pm - 8 pm)
Principle The Siemens Centaur assay is a two-site sandwich immunoassay using direct chemiluminometric technology. The insulin is standardized against the World Health Organization 1st IRP 66/304.
Interpretation Insulin is produced, stored and secreted by the beta cells of the islet of Langerhans. Insulin is released in response to glucose in blood postprandial. The half-life is 5-10 minutes. Insulin stimulates the uptake of glucose into the liver, adipose and muscle tissue. Low levels are observed in patients with diabetes mellitus have a deficiency of insulin production (Type I) or have tissues that are insulin resistance (Type II). High levels are observed in patients with an insulinoma. Insulin measurements are also useful for monitoring diabetics on oral hypoglycemic agents.
Container type gold top gel tube
Amount to Collect 2 mL
Sample type Blood
Normal range 3-25 mU/L (mcU/mL)
Stability 8 hours at room temperature
24 hours at 2-8°C.
freeze at -20°C if > 24 hours

Interferences Insulin therapy cross-reacts with antibodies used in the test. Therefore, the insulin test cannot be used to distinguish between endogenously produced insulin from exogenous administration. C-peptide can be measured to determine endogenous insulin secretion.
References 1. Tietz NW (ed). Fundamentals of Clinical Chemistry. Ed. 5. Philadelphia: WB Saunders, 377-378, 2001.

2. Chevenne D, Trivin F, Porquet D. Insulin assays and reference values. Diabetes Metab. 1999;25:459-76.

3. Bayer(TM) Clinical Methods Insulin Advia Centaur Operator's Guide. PN 2230141. Last accessed Oct 2004. Siemens Medical Solutions Diagnostics, Malvern, PA.
CPT coding 83525
Last Updated 10/22/2012 6:30:58 PM
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