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AST (Aspartate Amino Transferase)

Item Value
Available Stat? Yes
Test code AST
Performed by? Chemistry
Sendout? no
Price range $
In House Availability Routine: 7 days.
Principle AST activity is proportional to the rate of decrease in NADH, monitored by measuring absorbance at 340 nm. The addition of α-ketoglutarate initiates the reaction. Pyridoxal -5'-phosphate is NOT used in this method.
Interpretation 1. Increased in acute MI, liver disease, infectious mononucleosis, biliary obstruction, muscular diseases and trauma (IM injections), acute pancreatitis, 50% of cerebral infarcts, occasionally in cerebral neoplasm or renal infarction.

2. For differentiation of liver diseases, AST in acute viral hepatitis may be increased 14 times, while in obstructive jaundice, cirrhosis, or liver metastasis, it increases only 1-3 times.

3. Normal in angina pectoris, coronary insufficiency, congestive heart failure without liver damage.

Container type gel tube (gold or green top).
Amount to Collect 2 mL
Sample type Blood
Special instructions Moderately hemolyzed serum will not be assayed.
Normal range U/L
Age U/L Range
2 wks-2 yrs 9-80
2-60 yrs 10-41
60-90 yrs 10-48
>90 yrs 11-38

Synonyms Liver Panel (ALB, ALT, AST, ALKP, TP, TBIL, DBIL);Metabolic Comprehensive Panel (METC): METB, TBIL, TP, ALB, ALT, AST, ALKP;
Stability Serum stable 24 hrs at room temperature; 4 weeks at 4°C; 1 year at -20°C.
Interferences 1. Falsely elevated by opiates in patients with biliary tract disease or cholecystectomy (2.5-6.5 times).

2. Falsely decreased in diabetic ketoacidosis, beriberi, very severe liver disease, chronic hemodialysis.

3. Also elevated by cholinergics which cause spasm of sphincter of Oddi, and by theophylline, vitamins A and C, nicotinic acid, chlorpromazine, hydralazine, reserpine.

4 See also Young, D.S (reference #4).

Additional information Evaluation of liver, biliary, or myocardial damage.
References 1. Bayer(TM) Clinical Methods AST (57) B01-4139-01 Advia 1650 Operator's Guide. PN 073-5001-09. Last accessed June 2003. Siemens Medical Solutions Diagnostics, Malvern, PA.

2. Rodgerson, D.O., Osberg, I.M.: Sources of error in spectrometric measurement of aspartate aminotransferase and alanine amino - transferase activities in serum. Clin Chem 20:43, 1974.

3. Wolf, P.L., Williams, D., Coplon, N., Coulson, A.S.: Low aspartate transaminase activity in serum of patients undergoing chronic hemodialysis. Clin Chem 18:567, 1972.

4. Young, D.S. Effects of Drugs on Clinical Laboratory Tests. Ed. 4. Washington, D.C.: AACC Press; 1995.

5. Tietz, N.W. Textbook of Clinical Chemistry and Molecular Diagnosis, ed. 4. St. Louis, MO: Elsevier Saunders, 604-607; 2006.
CPT coding 84450
Last Updated 12/3/2008 12:00:46 AM
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