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HISTOPLASMA CAPSULATUM ANTIGEN DETECTION, URINE

Item Value
Approval req'd? yes, Microbiology Laboratory Medicine Resident
Available Stat? No
Test code HIAG
Performed by? Reference Laboratory - ARUP web page for Histoplasma Antigen by EIA, Urine test details.
Sendout? yes
Price range $$
Principle The Histoplasma antigen detection assay is an enzyme immunoassay to detect polysaccharide antigen in the urine of patients with H. capsulatum infection. This test is particularly useful for the detection of disseminated disease in immunocompromised patients.
Interpretation The sensitivity of the Histoplasma antigen assay varies with the severity of infection in the patient, as follows:

Disseminated ~ 95%
Acute pulmonary ~ 44%
Chronic pulmonary ~ 21%
Histoplasma meningitis ~ 67%


Specificity of this assay is ≥ 98% based on clinical diagnosis. Cross reactions may occur with Blastomyces dermatitidis and Paracoccidioides brasiliensis.
Container type sterile cup or tube
Amount to Collect 2 mL
Collection Instructions Not stable a room temperature. Refrigerate immediately. Transport to lab on ice.
Sample type Urine
Normal range Less than 2.0 EIA unit; interpreted as negative for Histoplasma antigen.
Stability 5 weeks at 4°C. Refrigerate immediately. Transport to lab on ice.
References 1. Wheat LJ, Kohler RB, Tewari RP. Diagnosis of disseminated histoplasmosis by detection of Histoplasma capsulatum antigen in serum and urine specimens. N. Eng. J. Med., 1996:314:83-8.

2. Sarosi GA, and Johnson PC. Disseminated histoplasmosis in patients infected with human immunodeficiency virus. Clin Infect Dis, 1992. 14 (Suppl 1):S60-S67.

3. Mandell GL, Bennett JE, and Dolin R., Eds. Principles and Practice of Infectious Diseases, 6th ed., Churchill Livingstone, Inc., NY, 2005.
CPT coding 87385
Last Updated 2/4/2013 4:14:05 PM
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