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Lab Manual for UCSF Clinical Laboratories

Lab Manual for SFGH

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HEMOGLOBINOPATHY EVALUATION

Item Value
Available Stat? No
Test code HGBV
Performed by? Chemistry
Sendout? no
Price range $$$
In House Availability Once a week, usually on Wednesdays. Requests for urgent testing between routine run dates will be considered individually, based on medical necessity. For approval call Lab Medicine Resident at 206-8593, or pager (415) 443-6969 after hours.
Principle Hemoglobins are separated and quantitated by an ion-exchange high performance liquid chromatography method (IE-HPLC, VARIANT II system, Bio-Rad). The method is based on electrical charge differences between hemoglobin molecules and the stationary phase in a chromatography column, resulting in distinct retention times for hemoglobin variants as they pass through the column.
Interpretation QUANTITATIVE RESULTS, INCLUDING HEMOGLOBIN A2, F:

* Hemoglobins A2, F, and S and C, if present, will be quantitated and reported. If MCV is below the reference range (< 80 fL), ZNPP (zinc protoporphyrin) will automatically be performed to evaluate for iron deficiency; the results will be reported.

* For quantitative A2, F order "Hemoglobinopathy Evaluation"

INTERPRETATION: For interpretation, consult the Laboratory Medicine Resident in Hematology (x65689; pager: 415-443-0179; pager, after hours and on weekends: 415-443-6969).
Container type EDTA (lavender top) tube
Amount to Collect 5 mL
Collection Instructions Mix tube well to avoid clotting.
Sample type Whole blood
Normal range
Adult: Hgb AA, Hgb A2 < 3.3 %, Hgb F < 2.0%
Newborn: Hgb FA, Hgb A2 < 3.3 %, Hgb F 60-90%

Synonyms Hemoglobin A2;Hemoglobin Electrophoresis, Qualitative;Hemoglobin F;
Additional information Include clinical history and order CBC. Both are required for interpretation. If no CBC is ordered, the lab will add this test.

CONFIRMATION OF ABNORMAL HEMOGLOBINS: If there is no prior record of an abnormal hemoglobin, the specimen will be sent out to a sendout lab for confirmation. Other confirmatory tests which may be performed include sickle cell solubility test and hemoglobin H preparation.
References 1. Bain, BJ: Haemoglobinopathy Diagnosis. Blackwell Science Ltd, 2001.

2. Waters et al.: An evaluation of the Bio-Rad Variant Haemoglobin Testing System for the detection of haemoglobinopathies. Clin Lab Haematol 20:31-40, 1998.

3. Instruction Manual for the Bio-Rad VariantªII ?-thalassemia Short Program. 702:037-2. Oct 2002. BioRad Laboratories, Hercules, CA.
CPT coding 83021
Last Updated 6/18/2011 8:31:42 AM
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