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HEMOGLOBIN S (SOLUBILITY TEST FOR SICKLE HEMOGLOBIN)

Item Value
Approval req'd? no
Available Stat? No
Test code HGBS
Performed by? Hematology
Sendout? no
Price range $
In House Availability Monday - Friday, 9:00 am - 3:00 pm
Principle A sickling hemoglobin in the deoxygenated (reduced) state is insoluble when placed in a high molarity phosphate buffer and will form polymers producing a turbid solution.
Interpretation Sickling hemoglobins S, C-Harlem, C-Ziguinchor, S-Travis, etc.) are insoluble and give a positive test. All other hemoglobins remain soluble.
Container type EDTA (lavender top) tube
Amount to Collect 1 mL
Sample type EDTA Whole blood
Normal range Negative
Stability Stable for 24 hours at room temperature (15-30°C) or 3 weeks at 2-10°C.
Additional information INDICATION: Screening test for sickling hemoglobins. It does not differentiate the heterozygous from the homozygous condition. Hemoglobin type must be further differentiated by electrophoresis or other methods.
References 1. Streck, Sickle-Chex Solubility Kit Package Insert 350430-7 (11/2004).

2. McGhee, DB. Structural Defects in Hemoglobin (hemoglobinopathies). In: Rodak, BF (ed.), Hematology – Clinical Principles and Applications, 2nd ed. Philadelphia, WB Saunders Co. 2002.
CPT coding 85660
Last Updated 11/8/2010 7:42:38 PM
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