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HEMOGLOBIN F STAIN, ACID ELUTION (KLEIHAUER BETKE TEST)

Item Value
Approval req'd? PRE-APPROVAL REQUIRED. M-F, 8 am-5 pm page (415) 443-0179. After hours, week ends, and holidays contact (415) 206-8590.
Available Stat? No
Test code FETS
Performed by? Hematology
Sendout? no
Price range $$
In House Availability Monday - Friday, 9 am - 3 pm
Principle Hemoglobin A is eluted from the erythrocytes in a dried blood smear by acidic citric acid-phosphate buffer, whereas Hgb F is not. The red cells containing Hgb F stain densely with erythrosin; those that contained only Hb A appear unstained.
Interpretation 1. The qualitative test may be ordered for patients known to have increased Hb F to distinguish hereditary persistence of Hb F (uniform, or pancellular, distribution in RBCs) from thalassemia (uneven, or heterocellular, distribution in RBCs).

2. The quantitative test is used in cases of Rh incompatibility, in order to identify and estimate the extent of fetal-maternal hemorrhage. This is accomplished by measuring the proportion of densely stained (fetal) erythrocytes in a smear prepared from maternal blood.
Container type EDTA (lavender top) tube
Amount to Collect 1 mL
Sample type EDTA Whole blood
Normal range
Quantitation: < 0.05% (SI Unit: < 0.0005)
Qualitative: Normal

Synonyms Fetal Hemoglobin Screen;Kleihauer Betke Test;
Stability 3 days (do not freeze).
References 1. Harmening, D. (ed): Clinical Hematology and Fundamentals of Hemostasis, 4th ed., F.A. Davis Co., Philadelphia, PA. 2002. 585-586.

2. Sure-tech diagnostic, Associates, Inc., St. Louis, MO. Fetal Hemoglobin. Procedure no. 101. Revised 1991.
CPT coding 85460
Last Updated 4/27/2011 3:27:51 PM
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