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COCCIDIOIDES IMMITIS COMPLEMENT FIXATION

Item Value
Available Stat? No
Test code COCF
Performed by? Reference Laboratory - ARUP web page for Coccidioides Antibody by CF.
Sendout? yes
Price range $
Principle Patient serum is reacted with coccidioidin antigen in the presence of complement. If antibody is present, complement will be bound to antigen antibody reaction and not be available to produce hemolysis with indicator system of sheep RBC-hemolysin.
Method Complement fixation
Interpretation Sixty percent of patients with symptomatic primary infection have a positive CF titer by 4 weeks and 80-90% by 8 weeks. A rising CF titer is consistent with increasing severity of disease or spread of disease to extrapulmonary sites. A declining titer is a favorable prognostic sign. Titers may vary from laboratory to laboratory because of differences in time for complement fixation incubation. A CF Titer of 1:8 or less may persist for years after the primary infection. CF antibodies in CSF confirm the diagnosis of coccidioidal meningitis. Patients with disseminated infection or AIDS with disseminated coccidioidomycosis may not have significant antibody levels.
Container type Gold top gel tube (red top non-gel tube is acceptable); sterile tube for CSF
Amount to Collect 3 mL blood or 1 mL CSF
Collection Instructions Do not do fungal skin test, e.g., coccidioidin or histoplasmin before collecting blood for fungal serological studies.
Sample type Blood; CSF
Synonyms Coccidioidomycosis serology;
Additional information REQUISITION: Blood/Serum - Main Laboratory. Include brief history age and date of onset on requisition
References Pappagianis D, and Zimmer BL. 1990. Serology of Coccidioidomycosis. Clin. Microbiol. Rev., 3:247-268.
CPT coding 86635
Last Updated 3/23/2016 4:43:25 PM
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