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Lab Manual for Moffitt-Long and Mount Zion

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Clinical Laboratories Reflexive Ordering

Clinical Laboratories Reflexive Ordering

The UCSF Medical Board recognizes that in some instances it is medically appropriate to perform reflex testing based on an initial laboratory result and in other instances reflex testing should be at the discretion of the ordering practitioner. In accordance with the UCSF Clinical Laboratory OIG Compliance Plan we approve the following reflex testing activity as proposed by the Laboratory Director. (Approved by UCSF EMB 12/18/07)

TEST ORDER REFLEX ACTION OPTION

BLOOD BANK

Antibody Screen If positive, testing initiated as required to identify the antibody. None, always reflexed
Direct Coombs Test If positive, initiates testing to determine if reactivity due to IgG or Complement on Red cells None, always reflexed

CHEMISTRY

Hemoglobinopathy evaluation If variant hemoglobin is detected by HPLC , hemoglobin electrophoresis is performed to characterize and classify the variant. None, always reflexed

CYTOGENETICS

Chromosome analysis If an abnormality is detected the Director will determine the appropriate additional studies to be performed to characterize the abnormality
Lab may be asked to omit or inform the clinician prior to performing reflex testing.

IMMUNOLOGY

Hep B Surface Ag
Confirmatory test is run all all positives at a separate charge
None, always reflexed
HIV Antibody If positive, confirmatory western blot is performed None, always reflexed
HTLV-I Ab Screen A specimen which is positive by EIA will be referred automatically to the State Viral Diseases Laboratory for confirmatory testing, each step of which will incur additional charges None, always reflexed
RPR (Routine or Prenatal) If positive, titer is performed but not billed. If positive TP-AB is performed to confirm and billed. Requisition allows RPR to be ordered w/out reflex TP-AB

MICROBIOLOGY

Bacterial Culture If bacteria are present they are identified and susceptibility testing is performed as appropriate. Requisition allows for omission of full ID and susceptibility testing
Cryptococcal Antigen If positive, specimen is titered at a separate charge. None, always reflexed
First positive sputum smear for Acid-Fast-Bacilli (AFB) Automatic reflex to State laboratory for Molecular probe testing for M. tuberculosis. None, always reflexed

NCPL

HIV, Rapid Antibody Screen If positive a confirmatory Western Blot will be performed None, always reflexed

VIROLOGY

Clostridium difficile Antigen Cell culture and neutralization for toxin will be performed if antigen is positive and billed separately. None, always reflexed
Respiratory Virus DFA If positive polyvalent screening stain, further testing done with specific stains for individual viruses Requisition allows user to select panel or individual viral DFA's

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