Item Value
Test name Peripheral Blood Culture
Available Stat? No
Turn around times Up to 6 days
In House Availability Daily, all shifts
Performed by Microbiology
Normal range No growth
Container type Paired blood culture bottles (BD BACTEC Plus Aerobic & Lytic Anaerobic bottles)
Amount to Collect Adults: 20 mL total (10 mL for each bottle)
Pediatrics: Collect blood sample amount according to weight:
< 1 kg = 1 mL for aerobic only (0.5 ml for neonates < 72h old)
1 – 5 kg = 2 mL total (1 mL for each bottle)
5 – 15 kg = 3 mL total (1.5 mL for each bottle)
15 – 40 kg = 6 mL total (3 mL for each bottle)
>40 kg = 10 mL total (5 mL for each bottle)
Collection Instructions 1. Adults:
Collect 2 sets of cultures from different sites.
Collect at least one set of cultures from a peripheral site.

2. Pediatrics:
Amount of blood depends on weight of patient (see weight based minimums).

Anaerobic sample should be sent only in the following circumstances: Outpatients, patients with immunodeficiency, malignancy or after bone or human stem cell transplant, patients with gastrointestinal disorder, or at physician's request due to concern for anaerobes. Sending only an aerobic specimen is sufficient for all other patient populations.

3. Cleanse venipuncture site:
For patients with NO contraindication for use of chlorhexidine products: Cleanse the venipuncture site with ChloraPrep Single Swabstick using a back and forth motion for 30 seconds. Allow it to air dry. NOTE: If skin is soiled, clean with 70% isopropyl alcohol before using ChloraPrep.

For patients with a contraindication for use of Chlorhexidine products, DO NOT USE CHLORAPREP & follow this procedure: Clean skin of venipuncture site with 60-second friction scrub of 70% isopropyl alcohol to a 5 cm circular area. Apply 10% PVP Iodine to venipuncture site skin in a circular motion to a 5 cm area starting in the center. Allow it to air dry. Following the venipuncture, remove residual iodine from patient's skin with 70% isopropyl alcohol.

4. Remove plastic cap of each bottle and scrub top of each bottle with 70% alcohol prep pad.

5. Perform venipuncture and obtain sample.
Nursing staff: Refer to Blood Culture Methods (General) procedure in Nursing Procedures Manual.
Clinical Labs Phlebotomy staff: Refer to Clinical Labs Blood Culture Collection procedure.

6. Instill sample into aerobic bottle first and then into anaerobic bottle. Do not aspirate air into the anaerobic bottle. Do not add more than 10 mL into each bottle. Gently invert bottles to mix contents.

7. Label each bottle with patient's name and medical record number and site of draw. Do not place label on neck of bottle or bottom (underneath) of bottle, and do not cover bar code on bottle with the label. Place label vertically on bottle.
Sample type Blood

If you have additional questions regarding this test, please call: 415-353-1667