Item Value
Test name Differential Time to Positivity
Available Stat? No
Turn around times 6 days
In House Availability Daily, all shifts
Performed by Microbiology
Normal range No growth
Container type Two sets of paired blood culture bottles (BD BACTEC Plus Aerobic and Lytic Anaerobic)
Amount to Collect Adults: 40 mL total (20 mL for each set / 10 mL for each bottle)
Pediatrics: Collect blood sample amount according to weight for EACH set:
< 1 kg = 1 mL for aerobic only (0.5 ml for neonates < 72h old)
1 – 5 kg = 2 mL total (1 mL for each bottle)
5 – 15 kg = 3 mL total (1.5 mL for each bottle)
15 – 40 kg = 6 mL total (3 mL for each bottle)
>40 kg = 10 mL total (5 mL for each bottle)
Collection Instructions For detailed instructions, see nursing manual.

Peripheral vein and CVC line samples must be obtained within 15 minutes of each other.

The volumes of blood obtained from the peripheral vein & from the CVC must match. (e.g. if only 12 mLs is obtained from peripheral stick, obtain only 12 mLs from CVC).

Draw peripheral blood sample first since the amount of blood obtained will determine how much is obtained from the CVC.

Inoculate one set of aerobic & anaerobic bottles with the peripheral sample, dividing the volume in half for each bottle. Label bottles as "peripheral" and submit with a microbiology requisition (if applicable) for a DTTP Peripheral Blood Culture.

Inoculate a second set of aerobic & anaerobic bottles with the line sample, dividing the volume in half for each bottle. Label bottles as "line" and submit with a microbiology requisition (if applicable) for a DTTP Central Blood Culture.

Put all 4 bottles and requisitions (if applicable) in the same specimen bag and send to the lab.
Sample type Whole blood

If you have additional questions regarding this test, please call: 415-353-1667