UCSF Navigation Bar

UCSF Departments of Pathology & Laboratory Medicine Home Page

Lab Manual for UCSF Clinical Laboratories

Lab Manual for SFGH

Internal Resources

Click here for more information about laboratory operations and procedures

If you have additional questions regarding this test, please call: 415-353-1667

Search Our Test Menu

Testosterone, Free (includes Total Testosterone), Pediatric

Item Value
Test Update Information Effective June 1, 2016 free testosterone testing will now be performed in-house at China Basin Chemistry. Results may change by up to 15% compared to those obtained using the previous method at Quest.
Approval req'd? Yes, contact Chemistry/Immunology Resident at 415-353-1438 for patients > 20 years old.
Available Stat? No
Test code FTCP
Test group Testosterone
Performed by China Basin Chemistry
In House Availability Test run on Thursday
Method Total testosterone by LC-MS/MS, free testosterone by calculation
Container type Red top (Gold top NOT acceptable )
Amount to Collect 2 mL (blood)
Sample type Serum
Preferred volume 1 mL (serum)
Min. Volume 0.75 mL (serum)
UCSF Rejection Criteria Collected in Gold top
Processing notes Refrigerate serum.
Units pg/mL (free) and ng/dL (total)
Normal range Testosterone, Total:
Age Males Females
Premature (26-28 weeks) 59-125 ng/dL 5-16 ng/dL
Premature (31-35 weeks) 37-198 ng/dL 5-22 ng/dL
Newborn 75-400 ng/dL 20-64 ng/dL
1-5 months 14-363 ng/dL < 20 ng/dL
6-24 months < 37 ng/dL < 9 ng/dL
2-3 years < 15 ng/dL < 20 ng/dL
4-5 years < 19 ng/dL < 30 ng/dL
6-7 years < 13 ng/dL < 7 ng/dL
8-9 years 2-8 ng/dL 1-11 ng/dL
10-11 years 2-165 ng/dL 3-32 ng/dL
12-13 years 3-619 ng/dL 6-50 ng/dL
14-15 years 31-733 ng/dL 6-52 ng/dL
16-17 years 158-826 ng/dL 9-58 ng/dL
18-39 years 300-1080 ng/dL 9-55 ng/dL



Total testosterone reference ranges by pubertal stage :

Tanner Stage Males Females
Tanner Stage I 2-15 ng/dL 2-17 ng/dL
Tanner Stage II 3-303 ng/dL 5-40 ng/dL
Tanner Stage III 10-851 ng/dL 10-63 ng/dL
Tanner Stage IV-V 162-847 ng/dL 11-62 ng/dL



Testosterone, Free:
Age Males Females
5 -9.9 years ≤ 5.3 pg/mL 0.2-5.0 pg/mL
10-13.9 years 0.7-52.0 pg/mL 0.1-7.4 pg/mL
14-17.9 years 18-111 pg/mL 0.5-3.9 pg/mL
18-69 years 35-155 pg/mL 0.1-6.4 pg/mL


Synonyms Free testosterone
Stability Refrigerated for 2 weeks, frozen at -20oC for 3 years.
Turn around times Test run once per week (Thursday). Turnaround time 2-8 days
Additional information Reference ranges adopted from Quest Diagnostics based on correlation studies comparing our method with the Quest method and by in-house testing of 24 normal male and 31 normal female volunteers in the UCSF Laboratory.

Includes determination of total testosterone.

Free: To convert pg/mL to pmol/L (SI units) multiply by 3.467.

Total: To convert ng/dL to nmol/L (SI units) multiply by 0.0347.
CPT coding 84402 and 84403
LOINC code 2991-8 and 2986-8
Last Updated 3/24/2017 12:35:09 PM
Entry Number 1731
UCSF home page UCSF home page About UCSF Search UCSF UCSF Medical Center