UCSF Navigation Bar

UCSF Departments of Pathology & Laboratory Medicine Home Page

Lab Manual for UCSF Clinical Laboratories

Lab Manual for SFGH

Internal Resources

Click here for more information about laboratory operations and procedures

If you have additional questions regarding this test, please call: 415-353-1667

Search Our Test Menu

Acanthamoeba Culture and smear

Item Value
Available Stat? No
Test code P417
Test group Parasitology
Performed by Microbiology
In House Availability Monday-Friday day shift
Method Culture, and exam of smear by microscopy
Collection Instructions Transport specimens to Microbiology as soon as possible.

Corneal scrapings or biopsy: Place specimen directly onto the center of a non-nutrient agar plate. Label the plates at the edge so as not to interfere with microscopic analysis. Tape the plate closed and transport in a sealed specimen bag. If a stain is requested, submit a slide with a centered dime-sized smear, frosted side face up, labeled, and in a slide transport container.

Contact lens solution: Submit in original container or sterile container.

Contact lens case: Submit with fluid or with lenses.
Container type Corneal scraping/biopsy: Non-nutrient agar plate, Contact lens solution: Sterile container, Contact lens case with solution or lens
CSF/Tissue: CSF tube or sterile collection tube
Amount to Collect Contact lens solution: 0.5 mL
CSF: 5 mL
Tissue: approximately 6 mm
Sample type Corneal scraping/biopsy
Contact lens solution
Contact lens case with fluid or lens
CSF
Brain tissue
Skin abscess
Preferred volume Contact lens solution: 0.5 mL
CSF: 5 mL
Tissue: 6 mm
Min. Volume Contact lens solution: 0.5 mL
CSF: 2 mL
Tissue: 3 mL
UCSF Rejection Criteria Dry contact lens case without contact lens
Processing notes Store sample at room temperature.
Notify a parasitologist or CLS when specimen arrives.
Normal range No Acanthamoeba spp. present/isolated
No Parasites Seen
Synonyms Acanthamoeba, Naegleria, Balamuthia, Amoeba
Turn around times Culture: 7-10 days, Smear: 1-3 days
Additional information A culture for Acanthamoeba will be performed on all specimen types.

An additional charge is billed for the smear.

For ophthalmology specimens, a smear is stained and examined when a slide is received, and when contact lens solution or contact lens case with fluid or lens is received.
CPT coding Culture: 87081, Smear:87207
Last Updated 6/5/2017 9:52:45 AM
Entry Number 1722
UCSF home page UCSF home page About UCSF Search UCSF UCSF Medical Center