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Trichomonas vaginalis RNA

Item Value
Approval req'd? No
Available Stat? No
Test code P715
Performed by Microbiology
In House Availability Test performed 3 times per week
Method Transcription Mediated Amplification
Collection Instructions Do not discard fluid in the collection tube. Do not use expired swab collection kits. Swab collection kits are available from Material Services (Unisex swab PMM# 399818, Vaginal swab PMM# 59046 ).
Endocervical swab: Use the APTIMA Unisex Swab Specimen Collection Kit
(white label on Swab Specimen Transport Tube).
1. Remove excess mucus from the cervical os and surrounding mucosa using the large, white
shaft cleaning swab. Discard this swab.
2. Insert the small blue shaft collection swab into the endocervical canal. Gently rotate the swab
clockwise for 10 to 30 seconds.
3. Withdraw the swab carefully, avoiding any contact with the vaginal mucosa.
4. Remove the cap from the swab specimen transport tube and immediately place the swab into
the transport tube.
5. Carefully break the swab shaft against the side of the tube at the score line and discard the
top portion of the swab shaft, using care to avoid splashing of contents.
6. Recap the swab specimen transport tube tightly, label and ship to the lab.
Vaginal swab: Use the APTIMA Vaginal Swab Specimen Collection Kit
(orange label on Vaginal Swab Transport Media tube)
1. Partially peel open the swab package. Remove the swab. Do not touch the soft tip or lay the
swab down. If the soft tip is touched, the swab is laid down, or the swab is dropped, use a
new APTIMA Vaginal Swab Specimen Collection Kit.
2. Hold the swab, placing your thumb and forefinger in the middle of the swab shaft covering the
score line. Do not hold the swab shaft below the score line.
3. Carefully insert the swab into the vagina about 2 inches (5 cm) past the introitus and gently
rotate the swab for 10 to 30 seconds. Make sure the swab touches the walls of the vagina so
that moisture is absorbed by the swab and then withdraw the swab without touching the skin.
4. While holding the swab in the same hand, unscrew the cap from the tube. Do not spill the
contents of the tube. If the contents of the tube are spilled, use a new APTIMA Vaginal Swab
Specimen Collection Kit
5. Immediately place the swab into the transport tube so that the score line is at the top of the
tube.
6. Carefully break the swab shaft against the side of the tube at the score line and discard the
top portion of the swab shaft, using care to avoid splashing of contents.
7. Recap the swab transport media tube tightly, label and ship to the lab.

Male urethral swab: Use the APTIMA Unisex Swab Specimen Collection Kit (white label on Swab Specimen Transport Tube).
1. The patient should not have urinated for at least 1 hour prior to sample collection.
2. Insert the small blue shaft collection swab (NOT the larger white shaft cleaning swab) 2-4 cm into the urethra. Gently rotate the swab clockwise for 2-3 seconds.
3. Withdraw the swab carefully.
4. Remove the cap from the swab specimen transport tube and immediately place the swab into the transport tube.
5. Carefully break the swab shaft against the side of the tube at the score line and discard the top portion of the swab shaft, using care to avoid splashing of contents.
6. Recap the swab specimen transport tube tightly, label and ship to the lab.
Container type Endocervical and male urethral: APTIMA Unisex swab collection kit; Vaginal: APTIMA Vaginal swab collection kit
Amount to Collect One swab; If C. trachomatis/N. gonorrhoeae testing also ordered, single swab may be sent for both CT/CG and Trichomonas testing
Sample type Endocervical, vaginal, male urethral
UCSF Rejection Criteria Specimen received >60 days after collection, no swab in fluid, no fluid in tube
Processing notes Male urethral swabs are not tested at UCSF. Give swab to supervisor to send to Quest (test code 90801)
Normal range Not detected
Synonyms Trichomonas
Stability Room temperature or refrigerated 60 days
Turn around times 1-3 days
CPT coding 87661
LOINC code 46154-1
Last Updated 9/23/2016 4:38:21 PM
Entry Number 1721
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