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PRU Test P2Y12 Reaction

Item Value
Approval req'd? No, but must contact Hematology at x3-1747 to receive collection kit. For Neurointerventional radiology only.
Available Stat? No
Test code CLOP
Performed by Hematology, Parnassus
In House Availability Monday - Friday 0800 - 1600
Collection Instructions Contact Hematology at x3-1747 for collection kits. DO NOT collect any specimens before 0800 or after 1600.

1. Whole blood samples must be collected in or immediately transferred to Greiner 2.0 mL partial fill blue top tubes containing 3.2% Sodium Citrate. The tube must be filled to its intended whole blood capacity (indicated by small black line).
2. Whole blood may be collected from venous sites using a 21 gauge or larger needle in an appropriate blood collection tube.
3. Blood samples should be obtained from an extremity free of peripheral venous infusions.
4. Collect a discard tube first (approximately 2 mL), Greiner partial fill blue tops 2nd, and a lavender for CBC last.
5. Gently invert the sample tube at least 5 times to ensure complete mixing of the contents.
6. Blood must set a minimum of 10 minutes after collection before testing but no longer than 4 hours.


Samples cannot be pneumatic tubed.

A PRU will be ordered in computer. If no CBC or CBCD is requested, Central Processing will order a hematocrit (HCT) and platelet count (PLT).
Container type
1. Greiner partial fill discard
2. Greiner partial fill 3.2% Sodium citrate blue top
3. Lavender EDTA tube


Amount to Collect 8 mL
Sample type Citrated and EDTA anticoagulated whole blood
Preferred volume 8 mL
Min. Volume 6 mL
UCSF Rejection Criteria Clotted samples or if stability period exceeded.

Incorrect tube type and/or no discard tube received.

Patients with Hct's < 33% and/or Plt's < 119 x 109/L
Processing notes Deliver immediately to Hematology
Units PRU
Normal range 182-335
Synonyms P2Y12, Plavix Reaction
Stability 4 hours
Turn around times 4 hours
Additional information The VerifyNow PRUTest is designed to measure platelet P2Y12 receptor blockade.

Patients with inherited platelet disorders such as von Willebrand Factor Deficiency, Glanzmann Thrombasthenia and Bernard-Soulier Syndrome have not been studied with the VerifyNow PRUTest. The VerifyNow PRUTest is not intended for use with these types of platelet disorders.

Patients who have been treated with Glycoprotein IIb/IIIa inhibitor drugs should not be tested until platelet function has recovered. This time period is approximately 14 days after discontinuation of drug administration for abciximab (ReoPro) and up to 48 hours for eptifibatide (Integrilin) and tirofiban (Aggrastat). The platelet function recovery time varies among individuals and is longer for patients with renal dysfunction.

Test performance was not affected by hematocrit values between 33-52%, or platelet count
values between 119,000 - 502,000/µL. Based on an in-house study performed on 3/29/16, correlation showed that as hematocrit increased, PRU results decreased.

CPT coding 85576
LOINC code 49010-2
Last Updated 11/29/2017 3:48:16 PM
Entry Number 1714
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