Zeiss Spinning Disk - Werb Scope
Colloquially known as "Werb Scope", the microscope is a Yokogawa style spinning disk confocal on a Zeiss Axiovert 200M base. Compared to a traditional laser scanning confocal microscope that uses a single pinhole to direct light onto a sample and then to a PMT, a spinning disk microscope uses a rotating array of microlenses and pinholes to excite many sample location, which are then projected upon a camera. Advantages of this design include increased light throughput (attributed to the microlenses) and decreased image acquisition time (attributed to the camera).

Werb Scope uses a 4-line (405nm, 488nm, 560nm, 640nm) fiber optic laser launch for excitation. The Andor Clara High Resolution camera is used for image collection.

Objectives include:

For access please follow the Request Usage directions.

Werb Spinning Disk User Guide (pdf)

Owners' Consortium:
Werb Lab
Mouse gallbladder. Image Credit: Claire O'Leary, PhD. Locksley Lab