Total Internal Reflection Fluorescence
Our through-the-objective TIRF microscope is based on an inverted Zeiss Axioscope body utilizing many of the automated components and a Dual-View system for simultaneous imaging.

The system runs on both Micro-Manager and Metamorph.

Laser lines currently consist of a 488 nm line and a 560 nm line. Additional imaging modalities include IRM and brightfield.

For access please follow the Request Usage directions.

TIRF User Guide (pdf)

Owners' Consortium:
Krummel Lab
Mouse OT1 Tcells. Image Credit: En Cai, PhD. Krummel Lab