Gen3 2-Photon Microscope
The "Gen3" is the third generation of custom 2-photon laser scanning  microscopes in the BIDC. The instrument claims two IR laser excitation sources; 1) 18W Coherent Chameleon Vision II (680nm - 1080nm); 2) 8W MaiTai Ti-Saphire (710nm - 920nm).

An array of 6 PMTs allows for the simulataneous detection of violet, blue, green, yellow, red, and far red dyes at video rate.

The system uses an upright geometry, an automated Prior XY stage, and a piezo Z drive. Automation is controlled by µMagellan. Additionally, there is a perfusion system with an in-line and stage heater and an anesthesia setup making the microscope suitable for slide, slice, explant, and intravital imaging.

The objective is an Olympus 25x LWD 1.05 NA water immersion.

For access please follow the Request Usage directions.

Gen3 User Guide (pdf)

Owners' Consortium:
Krummel Lab
Looney Lab
Verkman Lab

Mouse tumor. Image Credit: Kaitlin Corbin, BIDC